News Update on Molecular Identification Research: Aug – 2019

Molecular identification and isolation of the Waxy locus in maize

The Waxy (Wx) locus in maize determines the amylose content of spore and reproductive structure tissue. There ar many mutant alleles of the locus caused by insertion of exchangeable dominant components. within the gift study, we’ve used the properties of dominant component alleles to spot the Wx locus and its factor product, with the next objective of analytic  the weather inflicting the mutations. we tend to gift proof that the Wx locus encodes a starch granule-bound fifty eight kd peptide that’s synthesized in vitro as a sixty five kd precursor. we tend to describe the isolation of recombinant plasmids containing complementary DNA inserts homologous to Wx informational RNA and a recombinant λ virus containing a genomic Eco Little Rhody fragment encompassing most or all of the Wx transcription unit. [1]

Molecular identification of prey in predator diets

In several things prey alternative by predators within the field can not be established or quantified victimization direct observation. The remains of some prey could also be visually known within the guts and excretion of predators however not all predators ingest such onerous remains and even people who do consume them can also ingest soft‐bodies prey that leave no recognizable remnants. The result’s, at best, a biased image of prey alternative. a spread of molecular techniques and applications are reviewed that enable prey remains to be known, usually to the species and even stage level. These techniques, all of that are still in use, embody accelerator action, a spread of immunologic approaches victimization polyclonal and organism antibodies to discover supermolecule epitopes, and recently developed enzyme chain reaction (PCR)‐based strategies for police investigation prey deoxyribonucleic aci.  [2]

Analysis of Intestinal Flora Development in Breast-Fed and Formula-Fed Infants by Using Molecular Identification and Detection Methods

Background: a clear distinction between nursed and formula-fed newborn infants is that the development of the microorganism, thought-about to be of importance for defense against harmful micro-organisms and for the maturation of the viscus system. during this study, novel molecular identification ways were wont to verify the information obtained by ancient culture ways and to validate the culture freelance fluorescent in place conjugation (FISH) technique. [3]

Unexpected diversity of Anopheles species in Eastern Zambia: implications for evaluating vector behavior and interventions using molecular tools

The understanding of protozoal infection vector species in association with their bionomical traits is important for targeting malaria interventions and measurement effectiveness. several zoology studies trust morphological identification of mosquitoes, limiting recognition to visually distinct species/species teams. genus Anopheles species assignments supported ribosomal desoxyribonucleic acid ITS2 and mitochondrial DNA COI were compared to morphological identifications from Luangwa and Nyimba districts in Northern Rhodesia. The comparison of morphological and molecular identifications determined that interpretations of species compositions, insect powder resistance assays, host preference studies, entice effectualness, and Plasmodium infections were incorrect once mistreatment morphological identification alone. [4]

Isolation and Molecular Identification of Colletotrichum gloeosporioides from Infected Peanut Seeds

Peanut (Arachis hypogaea (L) cv. Margenta) seeds ar presently, considerably thought of as a vital supply of macromolecule and edible fat. Anthracnose caused by Colletotrichum gloeosporioides may be a plant unwellness from seed that reduces phanerogam emergence and seed germination. The morbific fungi that isolated from peanut were known victimisation ITS one and ITS four primer with enzyme chain reaction and NCBI information victimisation BlastN formula. From NCBI victimisation blastN analysed was ninety fifth establish with alternative Colletotrichum species in NCBI information search. The morbificity of Colletotrichum gloeosporioides on peanut seeds and seedlings at fourteen days when sowing result was inoculated with pathogenic fungi with thirty two.3% and management was eighty three. Hence, this study was experimented with the thing to reveal seed-borne infections on tropical peanut in Malaysia. [5]


[1] Shure, M., Wessler, S. and Fedoroff, N., 1983. Molecular identification and isolation of the Waxy locus in maize. Cell, 35(1), pp.225-233. (Web Link)

[2] Symondson, W.O.C., 2002. Molecular identification of prey in predator diets. Molecular ecology, 11(4), pp.627-641. (Web Link)

[3] Harmsen, H.J., Wildeboer–Veloo, A.C., Raangs, G.C., Wagendorp, A.A., Klijn, N., Bindels, J.G. and Welling, G.W., 2000. Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods. Journal of pediatric gastroenterology and nutrition, 30(1), pp.61-67. (Web Link)

[4] Unexpected diversity of Anopheles species in Eastern Zambia: implications for evaluating vector behavior and interventions using molecular tools
Neil F. Lobo, Brandyce St. Laurent, Chadwick H. Sikaala, Busiku Hamainza, Javan Chanda, Dingani Chinula, Sindhu M. Krishnankutty, Jonathan D. Mueller, Nicholas A. Deason, Quynh T. Hoang, Heather L. Boldt, Julie Thumloup, Jennifer Stevenson, Aklilu Seyoum & Frank H. Collins
Scientific Reports volume 5, Article number: 17952 (2015) (Web Link)

[5] Rajeendran, A., Nulit, R., Yien, C. Y. S. and Ibrahim, M. (2017) “Isolation and Molecular Identification of Colletotrichum gloeosporioides from Infected Peanut Seeds”, International Journal of Plant & Soil Science, 19(2), pp. 1-8. doi: 10.9734/IJPSS/2017/35838. (Web Link)

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