News Update on High Performance Liquid Chromatography Research: Aug – 2019

 News Update on High Performance Liquid Chromatography Research: Aug – 2019

Analysis of Polyamines in Higher Plants by High Performance Liquid Chromatography

A sensitive (0.01-1 nmol) methodology has been developed for the analysis of polyamines in higher plant extracts supported high performance liquid natural process (HPLC) of their benzoyl derivatives (Redmond, Tseng 1979 J Chromatogr 170: 479-481). Putrescine, cadaverine, agmatine, spermidine, spermine, and therefore the less common polyamines nor-spermidine and homospermidine may be utterly resolved by reverse section HPLC, isocratic extraction with methanol:water (64%, v/v) through a 5-μm C18 column, and detection at 254 nm. the tactic may be directly applied to crude plant extracts, and it’s not subject to interference by carbohydrates and phenolics. [1]

Analysis of bacterial menaquinone mixtures by high performance liquid chromatography

Menaquinone mixtures of microorganism origin were separated consistent with the chain length and also the degree of chemical action of the polyisoprenyl side‐chain by reversephase partition high performance liquid activity. Menaquinones will be measured simply and sensitively by the activity system delineated  here. [2]

A Microassay for 1,25-Dihydroxyvitamin D Not requiring High Performance Liquid Chromatography: Application to Clinical Studies

This report describes a microassay for one,25––dihydroxyvitamin D [1,25–(OH)2D] in plasma that doesn’t need high performance liquid natural process. The assay involves speedy extraction and preliminary purification on a C-18 Sep-Pak cartridge, followed by final purification on a silicon oxide Sep-Pak mistreatment hexane-isopropanol. Quantitation of one,25-(OH)2D is achieved employing a nonequilibrium assay using one,25-(OH)2D receptor from calf thymus. the tactic is sensitive to one.5 pg/tube, with B50 occurring at nine pg/tube and a helpful assay vary of one.5–40 pg/tube. The intra- and interassay coefficients of variation ar vi.5% and 11.5%, severally, and therefore the methodology is linear over a large vary of sample dilutions. additionally, this assay measures each one,25-(OH)2D2 and one,25-(OH)2D3 with equal affinity. [3]

Graphene nanoplatelets based matrix solid-phase dispersion microextraction for phenolic acids by ultrahigh performance liquid chromatography with electrochemical detection

A simple, fast and eco-friendly approach supported matrix solid-phase dispersion microextraction (MSPDM) followed by ultrahigh performance liquid natural action let alone chemical science detection (UHPLC-ECD) was given for the microextraction and determination of six phenoplast acids in a very plant preparation (Danshen tablets). The parameters that influenced the extraction performance of phenoplast acids were investigated and optimized. [4]

Detection of Dichlorvos Residue in Cowpea Grains, Six Months after Application Using High Performance Liquid Chromatography

In Kano State Nigeria, particularly at the noted Dawanau grains market, dichlorvos (DDVP: a pair of, 2-dichlorovinyl dimethyl phosphate), that could be a artificial insecticide insecticide is being wide used against hold on insect pests of cowpea grains by traders and merchants alike. High Performance Liquid activity (HPLC) screening victimization commercially on the market whole of such associate degree insecticide insecticide was administrated in an exceedingly research laboratory at Bayero University Kano in 2014. This was geared toward decisive the presence or absence of the insect powder within the treated and untreated grains of cowpea before, day one and 6 months when application. [5]

Reference

[1] Flores, H.E. and Galston, A.W., 1982. Analysis of polyamines in higher plants by high performance liquid chromatography. Plant physiology, 69(3), pp.701-706. (Web Link)

[2] Tamaoka, J., Katayama‐Fujimura, Y. and Kuraishi, H., 1983. Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. Journal of Applied Bacteriology, 54(1), pp.31-36. (Web Link)

[3] REINHARDT, T.A., HORST, R.L., ORF, J.W. and HOLLIS, B.W., 1984. A microassay for 1, 25-dihydroxyvitamin D not requiring high performance liquid chromatography: application to clinical studies. The Journal of Clinical Endocrinology & Metabolism, 58(1), pp.91-98. (Web Link)

[4] Graphene nanoplatelets based matrix solid-phase dispersion microextraction for phenolic acids by ultrahigh performance liquid chromatography with electrochemical detection
Li-Qing Peng, Ling Yi, Qiu-Cheng Yang, Jun Cao, Li-Jing Du & Qi-Dong Zhang
Scientific Reportsvolume 7, Article number: 7496 (2017) (Web Link)

[5] R. Yusuf, S., H. Lawan, S., S. Wudil, B. and Sule, H. (2018) “Detection of Dichlorvos Residue in Cowpea Grains, Six Months after Application Using High Performance Liquid Chromatography”, Asian Research Journal of Agriculture, 7(4), pp. 1-6. doi: 10.9734/ARJA/2017/37992. (Web Link)

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