Transformation of cotton (Gossypium hirsutum L.) via particle bombardment

Embryogenic suspension cultures of cotton (Gossypium hirsutum L.) were subjected to particle bombardment, wherever high density particles carrying cellular inclusion deoxyribonucleic acid were accelerated towards the embryogenic plant cells. The cellular inclusion deoxyribonucleic acid coating the particles encoded hygromycin resistance. One to 2 weeks following bombardment, embryogenic cotton cells were placed in proliferation medium containing one hundred μg/ml hygromycin. Clumps of tissue that grew within the presence of hygromycin were subcultured at denseness into contemporary hygromycin-containing proliferation medium. Following successive transfer of embryogenic tissue to development so germination media, plants were recovered from transgenic embryogenic tissue. [1]

A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome.

We use a close fragment length polymorphism (RFLP) map to analyze body organization and evolution in cotton, a disomic polyploid. About 46.2% of nuclear deoxyribonucleic acid probes find RFLPs identifying upland cotton and Gossypium barbadense; and 705 RFLP loci are assembled into forty one linkage teams and 4675 cM. The subgenomic origin (A vs. D) of most, and body identity of fourteen (of 26), linkage teams is shown. The A and D subgenomes show similar recombinational length, suggesting that repetitive deoxyribonucleic acid within the physically larger A subgenome is recombinationally inert. RFLPs are somewhat additional plentiful within the D subgenome. [2]

A Modified Hot Borate Method Significantly Enhances the Yield of High-Quality RNA from Cotton (Gossypium hirsutum L.)

The isolation of biologically active polymer from cotton (Gossypium hirsutum L.) is tough because of interference by high levels of endogenous phenolics, polysaccharides, and secondary metabolites. A changed hot bawl out procedure was developed to combat these cellular constituents throughout tissue blend, leading to the quantitative recovery of polymer appropriate for conjugation analysis, in vitro translation, and complementary DNA synthesis. The effectivity of many hot bawl out buffer adjuvants for the qualitative and quantitative recovery of leaf polymer was monitored by absorbance spectra, gel ionophoresis, protein, and complementary DNA synthesis. Of the buffer adjuvants evaluated, polyvinylpyrrolidone-40 (PVP-40) exhibited the one, most vital impact on the yield and quality of polymer isolated from cotton leaves, though inclusion of deoxycholate and/or Nonident-40 (NP-40) any increased the standard of the polymer. [3]

Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense

Allotetraploid cotton species (Gossypium hirsutum and Gossypium barbadense) have long been cultivated worldwide for natural renewable textile fibers. The draft ordering sequences of each species square measure offered however they’re extremely fragmented and incomplete1,2,3,4. Here we tend to report reference-grade ordering assemblies and annotations for G. hirsutum accession TX Marker-1 (TM-1) and G. barbadense accession 3–79 by group action single-molecule period sequencing, BioNano optical mapping and high-throughput body conformation capture techniques. Compared with previous assembled draft genomes1,3, these ordering sequences show respectable enhancements in adjacency and completeness for regions with high content of repeats like centromeres. [4]

Field Evaluation of Foliar Blend Micronutrient Fertilizer on Cotton (Gossypium hirsutum) Production in Katsina State, Nigeria

During the 2013 and 2014 cropping season, field experiments were conducted to judge the result of combined application of NPK plant food and FoliarBlend matter application on yield and growth of Cotton at Funtua (longitude 07° 19’E, latitude 11° 32’N), in Katsina State, Nigeria. This study was done as a irregular complete block style with 3 replications. The treatments were T1 (500 mls of FoliarBlend in twenty litres of water); T2 (20 kilo NPK/ha + five hundred mls of FoliarBlend in 20 litres of water); T3 (40 kg NPK/ha + 500 mls of FoliarBlend in 20 litres of water); T4 (60 kg NPK/ha + 500 mls of FoliarBlend in 20 litres of water) and management. Soil samples were collected for the determination of chemical and physical properties before planting. [5]

Reference

[1] Finer, J.J. and McMullen, M.D., 1990. Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant cell reports, 8(10), (Web Link)

[2] Reinisch, A.J., Dong, J.M., Brubaker, C.L., Stelly, D.M., Wendel, J.F. and Paterson, A.H., 1994. A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome. Genetics, 138(3), (Web Link)

[3] Wan, C.Y. and Wilkins, T.A., 1994. A modified hot borate method significantly enhances the yield of high-quality RNA from cotton (Gossypium hirsutum L.). Analytical biochemistry, 223(1), (Web Link)

[4] Reference genome sequences of two cultivated allotetraploid cottons, Gossypium hirsutum and Gossypium barbadense
Maojun Wang, Lili Tu, […]Xianlong Zhang
Nature Genetics volume 51, (Web Link)

[5] Nafiu, K. A., Chude, V. O. and Ezendu, C. O. (2017) “Field Evaluation of Foliar Blend Micronutrient Fertilizer on Cotton (Gossypium hirsutum) Production in Katsina State, Nigeria”, Journal of Experimental Agriculture International, 17(2), (Web Link)