Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.
The interaction of the substantive dye azo dye with intact beta-D-glucans provides the idea for a fast and sensitive assay system for microorganism strains possessing beta-(1 ends up in 4),(1 ends up in 3)-D-glucanohydrolase, beta-(1 ends up in 4)-D-glucanohydrolase, and beta-(1 ends up in 3)-D-glucanohydrolase activities. an in depth correspondence was determined between cellulolytic activity and beta-(1 ends up in 4)-D-glucanohydrolase and beta-(1 ends up in 4),(1 ends up in 3)-D-glucanohydrolase activities in isolates from the bovine breadbasket. [1]
Cellulolytic Systems in Insects
Despite the presence of the many carbohydrolytic activities in insects, their cellulolytic mechanisms are poorly understood. Whereas cellulase genes are absent from the genomes of fruit fly or bombycid, alternative insects like termites turn out their own cellulases. Recent studies exploitation molecular biological techniques have brought new insights into the mechanisms by that the insects and their microorganism symbionts digest polyose within the bowel. desoxyribonucleic acid sequences of cellulase and associated genes, likewise as physiological and morphological data regarding the biological process systems of cellulase-producing insects, could permit the economical use of plastic biomass as a property energy supply. [2]
Factors Affecting the Cellulolytic Activity of Rumen Contents
The cellulolytic activity of first stomach contents was assayed by mensuration losses in weight and strength of cotton yarn incubated in first stomach contents within the presence of dietary additives (barley, tallow) and at completely different pH values. The addition of barley depressed cellulolysis and also the titre of filter paper-degrading bacterium given that the pH was allowed to fall. Lowering the pH from seven.0 to 6.0 by addition of HCl nearly utterly smothered attack of cotton and greatly reduced the titre of filter paper-degrading bacterium. The layering of animal oil on cotton smothered attack of cotton however didn’t decrease the titre of filter paper-degrading bacterium. [3]
Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production
Cellulosic biofuel is that the subject of accelerating attention. the most obstacle toward its economic practicableness is that the intractableness of lignocellulose requiring great amount of catalyst to interrupt. many designed yeast strains are developed with cellulolytic activities to cut back the requirement for catalyst addition, however exhibiting restricted result. Here, we tend to report the sure-fire engineering of a cellulose-adherent baker’s yeast displaying four completely different synergistic cellulases on the cell surface. [4]
Measurement of Cellulolytic Potential of Cellulase Producing Bacteria
The bioconversion of polyose and hemicellulose to soluble sugars is vital for world stabilization and for a property human society. Here, many cellulolytic microorganism were found in soil, compost and animal waste suspension of our surroundings.
Bacillus spp. square measure aerobic cellulolytic microorganism. Here, 2 true bacteria strains 2414, 2579 (T) and their mixed culture used for activity the cellulolytic potential. [5]
Reference
[1] Teather, R.M. and Wood, P.J., 1982. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol., 43(4), pp.777-780. (Web Link)
[2] Watanabe, H. and Tokuda, G., 2010. Cellulolytic systems in insects. Annual review of entomology, 55. (Web Link)
[3] Stewart, C.S., 1977. Factors affecting the cellulolytic activity of rumen contents. Appl. Environ. Microbiol., 33(3), pp.497-502. (Web Link)
[4] Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production
Zhuo Liu, Shih-Hsin Ho, Kengo Sasaki, Riaan den Haan, Kentaro Inokuma, Chiaki Ogino, Willem H. van Zyl, Tomohisa Hasunuma & Akihiko Kondo
Scientific Reports volume 6, Article number: 24550 (2016) (Web Link)
[5] Katiyar, P., Srivastava, S. K. and Tyagi, V. K. (2018) “Measurement of Cellulolytic Potential of Cellulase Producing Bacteria”, Annual Research & Review in Biology, 27(4), pp. 1-9. doi: 10.9734/ARRB/2018/34410. (Web Link)
