Latest Research on Bacteria Isolated: Dec – 2019

Bacteria isolated after unsuccessful endodontic treatment in a North American population

Objective: the aim of this study was to work out the composition of the microbial flora present in teeth after the failure of passage therapy during a North American population. These results were then compared with those of the previous Scandinavian studies. Study Design: Fifty-four root-filled teeth with persistent periapical radiolucencies were selected for retreatment. After removal of the root-filling material, the canals were sampled with paper points, and by reaming of the apical dentin. Both samples were grown under aerobic and strict anaerobic conditions. Then the bacterial growth was analyzed. Results: The microbial flora was mainly of 1 to 2 strains of predominantly gram-positive organisms. [1]

Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds

A survey of antibiotic-producing bacteria from the microbial flora attached to seaweeds and therefore the study of their antibiotic capacities were administered. From 5 species of green and brown marine algae, 224 bacterial strains were isolated and tested for antibiotic production. a complete of 38 strains displayed antibiotic activity, withEnteromorpha intestinalis being the source of the very best number of producer strains. All epiphytic bacteria with antibiotic activity were assigned to thePseudomonas-Alteromonas group. Antagonism assays among the isolates demonstrated that every producer strain inhibits the expansion of the opposite producers, also as of some nonproducer strains also isolated from seaweeds. [2]

Azoreductase activity of anaerobic bacteria isolated from human intestinal microflora.

A plate assay was developed for the detection of anaerobic bacteria that produce azoreductases. With this plate assay, 10 strains of anaerobic bacteria capable of reducing azo dyes were isolated from human feces and identified as Eubacterium hadrum (2 strains), Eubacterium spp. (2 species), Clostridium clostridiiforme, a Butyrivibrio sp., a Bacteroides sp., Clostridium paraputrificum, Clostridium nexile, and a Clostridium sp. the typical rate of reduction of Direct Blue 15 dye (a dimethoxybenzidine-based dye) in these strains ranged from 16 to 135 nmol of dye per min per mg of protein. The enzymes were inactivated by oxygen. In seven isolates, a flavin compound (riboflavin, flavin adenine dinucleotide, or flavin mononucleotide) was required for azoreductase activity. [3]

Bacteria isolated from the cuticle of plant-parasitic nematodes attached to and antagonized the root-knot nematode Meloidogyne hapla

Plant-parasitic nematodes are related to specifically attached soil bacteria. to research these bacteria, we employed culture-dependent methods to isolate a representative set of strains from the cuticle of the infective stage (J2) of the root-knot nematode Meloidogyne hapla in several soils. The bacteria with the very best affinity to connect to J2 belonged to the genera Microbacterium, Sphingopyxis, Brevundimonas, Acinetobacter, and Micrococcus as revealed by 16S rRNA gene sequencing. Dynamics of the attachment of two strains showed fast adhesion in but two hours, and interspecific competition for attachment sites. [4]

Plasmid Profile of Multidrug Resistant Bacteria Isolated from Wound Swabs from Hospital Patients in Akure, Nigeria

A total of 347 clinical wound swabs were obtained in Ondo State Specialist Hospital. Isolated pure cultures of bacteria were subjected to varied morphology and biochemical tests. The isolates were identified using Bergey’s Manual of Systematic Bacteriology. Ten antibiotics (Oxoid UK) discs were wont to determine the drug sensitivity pattern of the bacterial isolates. Plasmids were extracted by alkaline lysis method and electrophoresis of the DNA was administered on a 0.8% agarose gel electrophoresis.  This finding revealed that, out of 501 bacterial isolates collected from 347 wound swabs of patients; Staphylococcus aureus was the foremost predominant (34.73%). Pseudomonas aeuroginosa (27.74%), Streptococcus pyogenes (15.57%), Klebsiella pneumoniae (8.18%), Proteus mirabilis (7.38%) and Escherichia coli (6.40%) were isolated respectively. [5]

Reference

[1] Hancock III, H.H., Sigurdsson, A., Trope, M. and Moiseiwitsch, J., 2001. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 91(5), (Web Link)

[2] Lemos, M.L., Toranzo, A.E. and Barja, J.L., 1985. Antibiotic activity of epiphytic bacteria isolated from intertidal seaweeds. Microbial Ecology, 11(2), (Web Link)

[3] Rafii, F.A.T.E.M.E.H., Franklin, W.I.R.T. and Cerniglia, C.E., 1990. Azoreductase activity of anaerobic bacteria isolated from human intestinal microflora. Appl. Environ. Microbiol., 56(7), (Web Link)

[4] Bacteria isolated from the cuticle of plant-parasitic nematodes attached to and antagonized the root-knot nematode Meloidogyne hapla
Olivera Topalović, Ahmed Elhady, Johannes Hallmann, Katja R. Richert-Pöggeler & Holger Heuer
Scientific Reports volume 9, (Web Link)

[5] Peter Oluwayinka, A. and Cecilia Oladayo, A. (2017) “Plasmid Profile of Multidrug Resistant Bacteria Isolated from Wound Swabs from Hospital Patients in Akure, Nigeria”, Asian Journal of Medicine and Health, 2(3), (Web Link)

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